Large-scale process for high purity taxol from bark extract of Taxus yunnanesis

Citation
J. Xue et al., Large-scale process for high purity taxol from bark extract of Taxus yunnanesis, J LIQ CHR R, 23(16), 2000, pp. 2499-2512
Citations number
19
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
10826076 → ACNP
Volume
23
Issue
16
Year of publication
2000
Pages
2499 - 2512
Database
ISI
SICI code
1082-6076(2000)23:16<2499:LPFHPT>2.0.ZU;2-N
Abstract
A large-scale separation of taxol from semi-purified bark extract of Taxus yunnanesis was investigated. The chromatographic behavior of taxol and two close eluting analogues, cephalomannine and 7-epi-10-deacetyltaxol, was sys tematically studied on a C-18 bonded phase column with different mobile pha ses in reverse phase mode. According to the notably different selectivity o f the methanol and acetonitrile with water in the mobile phase and the most important requirement of capacity in preparative chromatography, the optim um suitable mobile phase used in a large-scale isolation of taxol and 7-epi -10-deacetyltaxol on a preparative C-18 column was given. Cephalomannine wa s eliminated by ozonolysis and, then after, separated throughout a normal p hase silica column. The whole large-scale process for high purity taxol fro m the bark extract from Taxus yunnanesis consisted of a preliminary purific ation with Biotage FLASH 150i system based on a prepacked normal phase sili ca cartridge, followed by using a C-18 Nova-pak(TM) column in a Waters Prep LC(TM) 4000 preparative HPLC system.