Shoots and embryo-like structures regenerated from cultured flax (Linum usitatissimum L.) hypocotyl segments

The effect of growth regulators and culture media on regeneration of flax f rom hypocotyl explants were tested. Shoot differentiation was strongly infl uenced by genotype, concentration of TDZ, combination of BAP and NAA, The h ighest number of shoots was obtained with the fibre cultivar Alex using TDZ (0.25 mg L-1) on MS medium. Embryo-like structures were formed only on 2.4 -D (5 mg L-1) pretreated hypocotyl segments of cultivar Szegedi-30 (oil fla x). As a consequence of 2,4-D pretreatment, the embryo-like structures had poorly defined or fused cotyledons and no apparent shoot apices. Morphologi cally not well developed ELS did not convert to plants.