A significant number of human immunodeficiency virus epitope-specific cytotoxic T lymphocytes detected by tetramer binding do not produce gamma interferon

Citation
Pa. Goepfert et al., A significant number of human immunodeficiency virus epitope-specific cytotoxic T lymphocytes detected by tetramer binding do not produce gamma interferon, J VIROLOGY, 74(21), 2000, pp. 10249-10255
Citations number
43
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
74
Issue
21
Year of publication
2000
Pages
10249 - 10255
Database
ISI
SICI code
0022-538X(200011)74:21<10249:ASNOHI>2.0.ZU;2-N
Abstract
Despite the seemingly important role of cytotoxic T-lymphocyte (CTL) respon ses in human immunodeficiency virus (HIV) disease pathogenesis, their measu rement has relied on a variety of different techniques. We utilized three s eparate methodologies for the detection of CTLs in a cohort of HIV-infected individuals who were also human leukocyte antigen A2 (HLA-A2) positive. Am ong the different CTL assays, a correlation was seen only when the Gag epit ope-specific HLA A*0201-restricted tetramer assay was compared with the ELI SPOT assay performed after stimulation with the Gag epitope; however, this correlation was of borderline statistical significance. On average, the tet ramer reagent detected a 10-fold-higher number of cells than were seen to p roduce gamma interferon by the ELISPOT assay. The implications of this CTL assay comparison and the possibility of phenotypic differences in HIV-speci fic CD8(+) T lymphocytes are discussed.