Evidence for the in vivo generation of oxidatively modified epitopes in patients with atherosclerotic endothelium

Citation
Na. Le et al., Evidence for the in vivo generation of oxidatively modified epitopes in patients with atherosclerotic endothelium, METABOLISM, 49(10), 2000, pp. 1271-1277
Citations number
26
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
METABOLISM-CLINICAL AND EXPERIMENTAL
ISSN journal
00260495 → ACNP
Volume
49
Issue
10
Year of publication
2000
Pages
1271 - 1277
Database
ISI
SICI code
0026-0495(200010)49:10<1271:EFTIVG>2.0.ZU;2-U
Abstract
There is increasing evidence that autoantibodies (AAbs) against oxidatively modified low-density lipoprotein (LDL) are present in humans and may be de tected in fasting plasma. Using a standardized immunoassay for the detectio n of circulating revels of AAbs against malondialdehyde (MDA)-modified LDL, we examined the acute changes in AAb levels during postprandial lipemia in a group of men and women without (n = 28) and with (n = 17) normal endothe lium. The presence of atherosclerotic vessel is documented by clinical evid ence of coronary artery disease (CAD). In response to the oxidative stress associated with postprandial lipemia, statistically significant reductions in AAb levels were demonstrated at 2 and 4 hours postprandially by paired t test. In patients with atherosclerotic arterial wall, the mean AAb level w as reduced to 90.8% of fasting levels (P < .001) after 2 hours and to 90% a fter 4 hours (P < .01). This acute reduction in AAbs against MDA-LDL appear s to be unique to atherosclerotic patients and could not be demonstrated in young controls with healthy blood vessels. In nonatherosclerotic controls, the mean normalized levels during postprandial lipemia were not statistica lly different from baseline (104.5% at 2 hours and 104.6% at 4 hours). The transient reduction in AAb levels with postprandial lipemia in atherosclero tic patients could be reproduced in a subset of the CAD patients after sign ificant improvement in the lipid profile with weight loss. In patients with atherosclerotic disease, the transient reduction in the level of circulati ng AAbs reflects either an increased propensity to generate oxidatively mod ified epitopes or a reduced capacity to remove excess modified epitopes. Th ese data are the first in vivo demonstration of an acute change in the oxid ative process during postprandial lipemia. Copyright (C) 2000 by W.B. Saund ers Company.