Effects of antidepressants on gamma-aminobutyric acid- and N-methyl-D-aspartate-induced intracellular Ca2+ concentration increases in primary cultured rat cortical neurons
M. Takebayashi et al., Effects of antidepressants on gamma-aminobutyric acid- and N-methyl-D-aspartate-induced intracellular Ca2+ concentration increases in primary cultured rat cortical neurons, NEUROPSYCHB, 42(3), 2000, pp. 120-126
We investigated the effects of antidepressants on the intracellular Ca2+ co
ncentration ([Ca2+](i)) increases induced by gamma-aminobutyric acid (GABA)
or N-methyl-D-aspartate (NMDA) in primary cultured rat cortical neurons us
ing fluorescence imaging, Acute treatment with imipramine inhibited GABA- a
nd NMDA-induced increases in [Ca2+](i) in a concentration-dependent manner.
Doses of 30 mu M clomipramine, desipramine, amoxapine and maprotiline also
inhibited both the GABA- and NMDA-induced [Ca2+](i) increases significantl
y, Both inhibitory effects of the five major antidepressants on the GABA- o
r the NMDA-induced [Ca2+](i) increases were well-correlated, Imipramine cou
ld inhibit significantly high-K+-induced [Ca2+](i) increases, Our previous
study has already shown that the GABA-induced [Ca2+](i) increase involves a
similar pathway to high-K+-induced Ca2+ influx, In conclusion, imipramine
and several other antidepressants have acute inhibitory effects on the GABA
-, NMDA- and high-K+-induced [Ca2+](i) increases, suggesting that these inh
ibitory effects are not related to specific receptors, One possibility is t
hat these effects may be commonly mediated via part of the high-K+-induced
[Ca2+](i) pathway, Copyright (C) 2000 S. Karger AG,Basel.