Accumulation of 4-hydroxynonenal-modified proteins in hippocampal CA1 pyramidal neurons precedes delayed neuronal damage in the gerbil brain

It has been proposed that reactive oxygen species and lipid peroxidation ha ve a role in the delayed neuronal death of pyramidal cells:in the CAI regio n. To explore the in situ localization and serial changes of 4-hydroxy-2-no nenal-modified proteins, which are major products of membrane peroxidation, we used immunohistochemistry of the gerbil hippocampus after transient for ebrain ischemia with or without preconditioning ischemia. The normal gerbil hippocampus showed weak immunoreactivity for 4-hydroxy-2-nonenal-modified proteins in the cytoplasm of CAI pyramidal cells. 4-hydroxy-2-nonenal immun oreactivity showed no marked changes after preconditioning ischemia. In the early period after ischemia and reperfusion, there was a transient increas e of nuclear 4-hydroxy-2-nonenal immunoreactivity in CAI pyramidal neurons. In contrast, cytoplasmic immunoreactivity transiently disappeared during s ame period and then increased markedly from 8 h to seven days. One week aft er ischemia, 4-hydroxy-2-nonenal immunoreactivity was observed within react ive astrocytes in the CAI region. Early nuclear accumulation of 4-hydroxy-2 -nonenal in CA1 neurons may indicate a possible role in signal transduction between the nucleus and cytoplasm/mitochondria, while delayed accumulation of 4-hydroxy-2-nonenal-modified proteins in the cytoplasm may be related t o mitochondrial damage. We conclude that 4-hydroxy-2-nonenal may be a key mediator of the oxidative stress-induced neuronal signaling pathway and may have an important role i n modifying delayed neuronal death. (C) 2000 IBRO. Published by Elsevier Sc ience Ltd. All rights reserved.