Detection of single-stranded DNA in the hydropic vestibule after the direct injection of antigen into the endolymphatic sac of guinea pigs

Immunohistochemical study for single-stranded DNA (ssDNA) with vestibule of guinea pigs was performed after the injection of keyhole limpet hemocyanin (KLH) into the right endolymphatic sac. Endolymphatic hydrops became evide nt by expansion of the Reissner's membrane in the cochlea of all animals 1 day after the injection of KLH. Increased ssDNA expression was detected in the sensory epithelium and transitional area, while temporal bones in the c ontrol group did not show any ssDNA immunoreactivities. ssDNA is accompanie d with the apoptotic change in the vestibule. Our results suggest that apop totic changes could be involved in the hydropic vestibule and these phenome na lead inner ear disturbance as seen in endolymphatic hydrops. (C) 2000 El sevier Science Ireland Ltd. Ail rights reserved.