Lack of association between pregnancy-induced hypertension and familial thrombophilia in a Czech population

Objective To study prevalences and associations of five molecular variants - R506Q (Leiden mutation) of factor V, 4G/5G in the promoter region of the plasminogen activator inhibitor-1 (PAI-1) gene, 677C/T in methylene-tetrahy drofelate reductase (MTHFR), 20210G/A in 3'UTR of the prothrombin gene and a 5' control region pentanucleotide repeat (TTTTA) in apo(a)- with pregnanc y-induced hypertension (PIH) in a Czech population. Methods Seventy-two women with PIH and 100 controls participated in the stu dy. Polymorphisms were detected by the polymerase chain reaction and differ ences in allele frequencies were tested by Fisher's exact test and the Mann -Whitney test. Results Allele frequencies of 506Q, 677T, 20210A and 4G PAI-1 were 5.6, 30. 6, 1.4 and 52.1%, respectively in the PIH group, and 3.5, 40.0, 2.5 and 45. 0%, respectively, in the control group. Frequencies of the particular allel es of apo(a) (7-11 repeats, respectively) were 0.0, 72.2, 13.2, 12.5 and 2. 1%, respectively, in the PIH group, and 0.5, 70.0, 16.0, 13.0 and 0.5%, res pectively, in the control group. No statistically significant association o f any of the five polymorphisms with PIH was found (p > 0.05). Observed ind ividual genotype combinations of R506Q, 677C/T and 20210G/A did not differ significantly between the PIH and the control groups (p > 0.05). Conclusion Genetic variants leading to thrombophilia probably do not much i ncrease the risk of PIH development.