Hydrolytic enzyme production in solid-state fermentation by Aspergillus niger 3T5B8

A mixture containing polygalacturonase, cellulase, xylanase and protease en zymes was produced using Aspergillus niger 3T5B8 on different agroindustria l residues by solid-state fermentation and tested for vegetable oil extract ion. The enzymic activities were evaluated using second-order empirical mod els from experimental data as a function of fermentation time and cellobios e concentration in the fermentation medium. The use of wheat bran as substr ate without cellobiose addition and 42 h of fermentation were the most favo urable conditions for producing the mixture of hydrolytic enzymes (polygala cturonase 30.75 U/ml, xylanase 30.62 U/ml, protease 5.27 U/ml). Another fav ourable condition was obtained when mango peel was used as substrate in the presence of 0.2% cellobiose and 24 h of fermentation (FPCAse 3.5 U/ml, xyl anase 20.33 U/ml). Enzymic formulations with suitable polygalacturonase and FPCAse activities were favourable to extract oil from the pulp of tropical fruits and oleaginous seeds. (C) 2000 Published by Elsevier Science Ltd. A ll rights reserved.