Isolation and analysis of DNA from museum specimens of butterflies (Lepidoptera, Papilionidae) with the aid of polymerase chain reaction using arbitrary and universal gene-specific primers
Ev. Zakharov et al., Isolation and analysis of DNA from museum specimens of butterflies (Lepidoptera, Papilionidae) with the aid of polymerase chain reaction using arbitrary and universal gene-specific primers, RUSS J GEN, 36(9), 2000, pp. 1017-1024
The effect of the duration of storage of entomological material on DNA pres
ervation was estimated. The results of the optimization of conditions for t
he analysis of random amplified polymorphic DNA in a polymerase chain react
ion (RAPD-PCR) are presented as applied to the DNA of lepidopterans of the
family Papilionidae. RAPD patterns are shown for the first time in Atrophan
eura alcinous and four species of the genus Parnassius (sensu late). The ap
plicability of museum specimens of butterflies for RAPD analysis was demons
trated. The results of PCR analysis using DNA obtained from different colle
ction specimens stored for up to five years were compared. The authenticity
of DNA obtained from collection specimens was proved using PCR with univer
sal primers, which are specific to the COI and COII cytochrome genes of mit
ochondrial DNA (mtDNA). The lengths of individual gene fragments obtained b
y the amplification of both museum and live specimens were 800 and 1600 bp.
The conservative regions of mitochondrial genome were shown to be slightly
different in two A. alcinous subspecies.