'Gelozymes' in organic synthesis: synthesis of enantiomerically pure (S)-2-hydroxy-(3-phenoxy)phenylacetonitrile with lipase immobilised in a gelatinmatrix

Authors
Fadnavis, NW Babu, RL Sheelu, G Deshpande, A
Citation
Nw. Fadnavis et al., 'Gelozymes' in organic synthesis: synthesis of enantiomerically pure (S)-2-hydroxy-(3-phenoxy)phenylacetonitrile with lipase immobilised in a gelatinmatrix, TETRAHEDR-A, 11(16), 2000, pp. 3303-3309
Citations number
10
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON-ASYMMETRY
ISSN journal
09574166 → ACNP
Volume
11
Issue
16
Year of publication
2000
Pages
3303 - 3309
Database
ISI
SICI code
0957-4166(20000825)11:16<3303:'IOSSO>2.0.ZU;2-0
Abstract
Lipase from Pseudomonas cepacia (Amano PS and PS Lipase, Fluka) immobilised in microemulsion-based organogels formed by gelatin solubilisation and cro sslinking with glutaraldehyde ('Gelozyme') has been used for the alcoholysi s of the butanoate ester of racemic 2-hydroxy-(3-phenoxy)phenylacetonitrile with 1-butanol in hexane to obtain (S)-2-hydroxy-(3-phenoxy)phenylacetonit rile. The immobilised enzyme can be used over 25 days (25 cycles) without s ignificant loss of enzyme activity (<10%). (C) 2000 Elsevier Science Ltd. A ll rights reserved.