'Gelozymes' in organic synthesis: synthesis of enantiomerically pure (S)-2-hydroxy-(3-phenoxy)phenylacetonitrile with lipase immobilised in a gelatinmatrix
Nw. Fadnavis et al., 'Gelozymes' in organic synthesis: synthesis of enantiomerically pure (S)-2-hydroxy-(3-phenoxy)phenylacetonitrile with lipase immobilised in a gelatinmatrix, TETRAHEDR-A, 11(16), 2000, pp. 3303-3309
Lipase from Pseudomonas cepacia (Amano PS and PS Lipase, Fluka) immobilised
in microemulsion-based organogels formed by gelatin solubilisation and cro
sslinking with glutaraldehyde ('Gelozyme') has been used for the alcoholysi
s of the butanoate ester of racemic 2-hydroxy-(3-phenoxy)phenylacetonitrile
with 1-butanol in hexane to obtain (S)-2-hydroxy-(3-phenoxy)phenylacetonit
rile. The immobilised enzyme can be used over 25 days (25 cycles) without s
ignificant loss of enzyme activity (<10%). (C) 2000 Elsevier Science Ltd. A
ll rights reserved.