Leukapheresis components may be cryopreserved at high cell concentrations without additional loss of HPC function

Citation
E. Cabezudo et al., Leukapheresis components may be cryopreserved at high cell concentrations without additional loss of HPC function, TRANSFUSION, 40(10), 2000, pp. 1223-1227
Citations number
19
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
TRANSFUSION
ISSN journal
00411132 → ACNP
Volume
40
Issue
10
Year of publication
2000
Pages
1223 - 1227
Database
ISI
SICI code
0041-1132(200010)40:10<1223:LCMBCA>2.0.ZU;2-5
Abstract
BACKGROUND: The aim of this study was to assess the feasibility of freezing mobilized peripheral blood progenitor cell (PBPC) components at higher cel l concentrations than are classically recommended for bone marrow. This app roach might have potential benefits, such as lower cost of processing and s torage and less risk of the complications associated with the transfusion o f large component volumes and large quantities of DMSO. STUDY DESIGN AND METHODS: In the first phase, small aliquots of 19 apheresi s components were cryopreserved at standard and higher cell concentrations (Aliquots A and B, respectively). In the second phase, 21 apheresis compone nts were split into two bags each and frozen at standard (Bag A) and high ( Bag B) cell concentrations. The differences in viability, cloning efficienc y, and nucleated cell recovery in Bags A and B were examined. Finally, the hematologic recovery of 10 patients who underwent autologous transplantatio n with PBPC components frozen at high cell concentrations was analyzed. RESULTS: The median cell concentration at freezing was 94 (57-100) x 10(6) per mL and 291 (220-467) x 10(6) per mi for Aliquots A and B, respectively, and 90.9 (45.4-92) x 10(6) per mi and 332 (171-582) x 10(6) per mt for Bag s A and B, respectively. The viability was significantly lower in samples f rozen at higher cell concentrations: 92 versus 83 percent (p = 0.001) and 8 7 versus 77 percent (p<0.001) for Aliquots and Bags A and B, respectively. Significant differences were not observed in the recovery of total nucleate d cells (102 vs. 101% and 98 vs. 105%) or the cloning efficiency after thaw ing (13 vs. 16% and 27 vs. 23%) for Aliquots and Bags A and B, respectively . The time to granulocyte engraftment greater than or equal to 0.5 x 10(9) per L and platelet engraftment greater than or equal to 20 x 10(9) per L wa s 9 (8-11) and 10.5 (7-21) days, respectively. CONCLUSION: The cryopreservation of PBPC components at standard concentrati ons and 3.3 (1.8-6.2)-fold cell concentrations has no adverse effect on the function of HPCs after thawing.