Localization and migration of benzimidazole resistant and susceptible adult Cooperia curticei in the small intestine of sheep after treatment with thiabendazole

Four groups of three lambs per group were experimentally infected with Coop eria curticei susceptible (two groups) or resistant (two groups) to benzimi dazoles, and distributions of adult worms in the small intestine were studi ed. For each Cooperia isolate, one group was treated with thiabendazole (TB Z) (5 or 50 mg/kg bodyweight) 28 days after infection. In the two untreated groups, the population of C. curticei were present from the second to the tenth meter of intestine from the pylorus with a maximum in the sixth meter for both isolates. After treatment with TBZ, the size of the resistant wor m population did not significantly decrease but a large number of worms wer e found towards the proximal sections of the intestine. In contrast, the su sceptible population was reduced by about 40% but the surviving worms remai ned at this same site of predilection after treatment. Measurements of the concentration of TBZ and 5OH-thiabendazole (5OH-TBZ) in the intestinal segm ents do not indicate a clear relationship between the localization of worms and TBZ or 5OH-TBZ concentrations at least 12h after the anthelmintic trea tment. The hypothesis of an enhanced expression of the mechanisms of resist ance in the first few meters of small intestine is suggested. (C) 2000 Else vier Science B.V. All rights reserved.