Immunodetection of PR-1-like proteins in grapevine leaves infected with Oidium tuckeri and in elicited suspension cell cultures

Authors
Repka, V Kubikova, J Fischerova, I
Citation
V. Repka et al., Immunodetection of PR-1-like proteins in grapevine leaves infected with Oidium tuckeri and in elicited suspension cell cultures, VITIS, 39(3), 2000, pp. 123-127
Citations number
34
Categorie Soggetti
Agriculture/Agronomy
Journal title
VITIS
ISSN journal
00427500 → ACNP
Volume
39
Issue
3
Year of publication
2000
Pages
123 - 127
Database
ISI
SICI code
0042-7500(200009)39:3<123:IOPPIG>2.0.ZU;2-9
Abstract
Three pathogenesis-related (PR-1-like) proteins extractable at pH 8.0 were found to accumulate in grapevine leaves after fungal pathogen (Oidium tucke ri) infection. These proteins were called gPR-1 (grapevine pathogenesis-rel ated) proteins. Estimated molecular masses in SDS-containing gels were: gPR -1a 15.5 kDa; gPR-1b 16.8 kDa; gPR-1c 17.7 kDa. Antiserum raised against to bacco PR-la reacted specifically with grapevine counterparts. Likewise, sti mulation of gPR-1 protein accumulation was observed when a set of prototype elicitors was added to grapevine cell suspension cultures. Results with in ducing elicitors also showed that the extracellular PR-1-like proteins repr esent the only isoforms of this prominent group of pathogenesis-related pro teins found in grapevine.