Citation
C. Ferrarello et al., Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography, ANALUSIS, 28(5), 2000, pp. 351-357
Citations number
24
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALUSIS
ISSN journal
03654877
→ ACNP
Volume
28
Issue
5
Year of publication
2000
Pages
351 - 357
Database
ISI
SICI code
0365-4877(200006)28:5<351:ISORLM>2.0.ZU;2-5
Abstract
The analytical performance of the Fast - Protein Liquid Chromatography (FPL
C) (on a Mono Q HR 5/5 column) and the conventional Anion-Exchange Chromato
graphy (on a Protein Pak DEAE 5PW column) to realise rabbit liver metalloth
ioneins separations and Cd speciation has been investigated and the results
are compared. The Mono Q column was found to provide higher resolution and
reproducibility in shorter separation times than Protein Pak DEAE 5PW, a c
onventional anion-exchange column. Thus, FPLC is proposed as a very useful
tool for the study of the polymorphism of metallothioneins.