New monoclonal antibody for the sensitive detection of hydroxy-s-triazinesin water by enzyme-linked immunosorbent assay

This article presents a new and sensitive immunoassay for the triazine meta bolites hydroxypropazine, hydroxyatrazine and hydroxysimazine. The assay is based on covalent immobilisation of antigen in combination with an enzyme- labelled anti-hydroxy-s-triazine monoclonal antibody. This system enabled t he development of an assay with variation coefficients below 3% and a detec tion limit below 0.01 mug/l hydroxyatrazine and hydroxypropazine. Analysis of hydroxyatrazine-spiked water of three different types yielded an average recovery of 102% at 0.1 mug/l hydroxyatrazine. Relative to hydroxyatrazine , assay cross-reactivity was 148% towards hydroxypropazine and 67% towards hydroxysimazine. (C)2000 Elsevier Science B.V. All rights reserved.