Integrated nuclear fluorescence and expression of hormone-binding sites inmalignant pleural effusions

OBJECTIVE: To investigate potential disease- and prognosis-associated nucle ar and cellular features from cell properties in a prospective study on mal ignant pleural effusions. STUDY DESIGN: Integrated nuclear fluorescence and the expression of binding capacities of carrier-immobilized estradiol, progesterone and testosterone ; and of labeled sarcolectin; and the presence of calcyclin were measured i n 50 cases with proven malignant pleural effusions (10 rnesotheliomns, 40 m etastasizing tumors). A double fluorescence technique using the fluorochrom e DAPI and a Texas Red-based avidin-biotin detection system were applied. D etailed clinical data, including the follow-up for up to 40 months, were in cluded. RESULTS: Pleural effusions in all patients with mesotheliomas occurred prio r to (9/10) or at the time of histologic confirmation. Mesotheliomas had th e highest tumor cell fraction (12.4%) in S phase and breast carcinomas the lowest (10.7%). More than 80% of malignant cells expressed binding capaciti es for the applied probes. A statistically significant correlation was note d between the S-phase-related turner cell friction and the expression of pr ogesterone receptors. Survival was associated with tumor origin, treatment by pleurodesis, and certain cytometric and histochemical features. CONCLUSION: The immunofluorescence double-staining technique can be applied successfully in malignant effusions to combine DNA measurements with those of immunohistochemical and ligand histochemical reactivity.