Isolation of n-Decyl-alpha(1 -> 6) isomaltoside from a technical APG mixture and its identification by the parallel use of LC-MS and NMR spectroscopy

Authors
Billian, P Hock, W Doetzer, R Stan, HJ Dreher, W
Citation
P. Billian et al., Isolation of n-Decyl-alpha(1 -> 6) isomaltoside from a technical APG mixture and its identification by the parallel use of LC-MS and NMR spectroscopy, ANALYT CHEM, 72(20), 2000, pp. 4973-4978
Citations number
14
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICAL CHEMISTRY
ISSN journal
00032700 → ACNP
Volume
72
Issue
20
Year of publication
2000
Pages
4973 - 4978
Database
ISI
SICI code
0003-2700(20001015)72:20<4973:ION-6I>2.0.ZU;2-4
Abstract
The identification of n-decyl alpha(1-6)isomaltoside as a main component of technical alkyl polyglucoside (APG) mixtures by the parallel use of liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (N MR) spectroscopy is described. Following enrichment on a styrene-divinylben zene-based solid-phase extraction material, unknown components were separat ed by reversed-phase liquid chromatography(LC). Chemical characterization w as achieved by both mass spectrometry and NMR spectroscopy. It is demonstra ted that the combination of LC-MS with various NMR techniques is very suita ble for stereochemical assignment of unknown components in technical APG mi xtures.