T lymphocytes mediate leaflet destruction and allograft aortic valve failure in rats

Background. Allograft heart valves are commonly used in cardiac surgery but ultimately fail. This situation is most acute in children. This study addr esses the role of T cell-mediated immune damage in allograft valve failure. Methods. Syngeneic (Lewis to Lewis) or allogeneic (Brown Norway to Lewis) a ortic valve grafts were implanted infrarenally into Lewis rat recipients (n = 24). Allogeneic valve grafts were also implanted into T cell-deficient r ats (nude; n = 12). At 7 14, and 28 days the valves were explanted and exam ined for structural integrity and cellular infiltration. Results. Syngeneic grafts maintained normal leaflet structure with little l eaflet immune infiltration. Allografts showed leaflet infiltration (7 days) , significant leaflet thickening, progressively decreased cellularity (14 d ays), and leaflet destruction (28 days). Infiltrates contained CD43+, CD3+, and CD8+ cells. Allografts in T cell-deficient rats showed none of the abo ve changes and maintained normal structural integrity. Conclusions. Allograft heart valves in the rat model undergo T cell-mediate d immune rejection, resulting in structural failure. (C) 2000 by The Societ y of Thoracic Surgeons.