Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen

We have examined the expression in bovine adrenal medulla of double C2 prot ein (DOC2), a vesicular protein which associates with intracellular phospho lipid and Ca2+ and is implicated in the modulation of regulated exocytosis. Extensive reverse transcription-PCR, Northern blot analyses and in vitro t ranslation reactions have been combined with immunological studies to provi de data to suggest that neither DOC2 alpha: nor DOC2 beta is expressed at d etectable levels in bovine adrenal chromaffin cells, and that a widely used monoclonal antibody directed against DOC2 also recognizes mitochondrial co mplex III core protein 2.