Highly sensitive and rapid detection of antibody catalysis by luminescent bacteria

Authors
Shulman, H Eberhard, A Eberhard, C Ulitzur, S Keinan, E
Citation
H. Shulman et al., Highly sensitive and rapid detection of antibody catalysis by luminescent bacteria, BIOORG MED, 10(20), 2000, pp. 2353-2356
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
10
Issue
20
Year of publication
2000
Pages
2353 - 2356
Database
ISI
SICI code
0960-894X(20001016)10:20<2353:HSARDO>2.0.ZU;2-S
Abstract
A highly sensitive, inexpensive, and facile bioluminescent assay for the de tection of catalytic antibodies has been developed. This assay may be used for the early detection of antibody catalysis. The efficiency of this techn ique was exemplified by the use of the luminescent bacterium VhM42 for moni toring an antibody-catalyzed retroaldol fragmentation reaction with aldolas e antibodies 38C2 and 24H6. (C) 2000 Elsevier Science Ltd. All rights reser ved.