Antimicrobial and biological effects of BOMPHOS and PHOMPHOS on bacterial and yeast cells

In this study, the antimicrobial effects of monophosphazenes such as SM, BO MPHOS, and PHOMPHOS were examined on bacterial and yeast strains. In additi on, the biological effects of these compounds were tested on the Saccharomy ces cerevisiae and Candida albicans cells. The SM has an antimicrobial effe ct on the bacterial and yeast strains within the range of 100 and 1500 mug. When the concentration was increased, the inhibition zone expanded on the growth media (P < 0.01; P < 0.001). Like SM, BOMPHOS molecule has antimicro bial activity on the bacterial and yeast cells. The most effective concentr ations of BOMPHOS on the microoganisms were observed by 1500 mug (P < 0.001 ). The PHOMPHOS did not effect on the bacterial and yeast cells between 100 and 1000 range, but it has an antimicrobial effect in 1500 <mu>g. In vitro media, the biological effects of these molecules were compared with vitami n E, melatonin, and fish oil on the yeast cells. In S. cerevisiae growth me dia, the cell densities were increased SM, BOMPHOS, and PHOMPHOS after 20, 30, and 45 h, The highest increase in the cell density were observed in med ia of BOMPHOS. In C. albicans growth media, the cell density was increased by melatonin after 20, 30, and 45 h, but were decreased by other supplement al groups. Lipid level of S. cerevisiae was reduced by administered 300 and 1000 pg vitamin E and fish oil (P < 0.01). In addition, the lipid level of the same yeast cell were diminished by the 1000 <mu>g melatonin and 300 mu g PHOMPHOS (P < 0.05, P < 0.01). The lipid level of C. albicans were increa sed by vitamin E and BOMPHOS and fish oil, but was decreased with PHOMPHOS (P < 0.01). In conclusion, while high concentration of PHOMPHOS has antimic robial effects on the bacterial and yeast cells, the SM and BOMPHOS have an timicrobial effects in all the concentrations. PHOMPHOS decreased the lipid lever of C. albicans, but BOMPHOS increased in the the same yeast cell. In addition, the antioxidants such as vitamin E, melatonin, and fish oils hav e affected on the lipid synthesis of yeast cells. (C) 2000 Academic Press.