Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: influence of calcium chloride, hemicellulase and theophylline

Authors
Pitta-Alvarez, SI Spollansky, TC Giulietti, AM
Citation
Si. Pitta-alvarez et al., Scopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: influence of calcium chloride, hemicellulase and theophylline, BIOTECH LET, 22(20), 2000, pp. 1653-1656
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
22
Issue
20
Year of publication
2000
Pages
1653 - 1656
Database
ISI
SICI code
0141-5492(200010)22:20<1653:SAHPBH>2.0.ZU;2-C
Abstract
CaCl2 (50 mM) and hemicellulase (0.5 U mg(-1)) increased the intracellular accumulation (60-250%), release (60-200%) and production (45-200%) of hyosc yamine and scopolamine in hairy roots of Brugmansia candida. Theophylline ( 0.25 mM), alone or in combination with hemicellulase, was ineffective in in creasing hyoscyamine and scopolamine production.