Allogeneic whole-tumour cell vaccination in the rat model of prostate cancer

Objective To investigate cancer immunotherapy using whole allogeneic (diffe ring tissue-type) tumour cells as vaccines in the rat prostate cancer model . Materials and methods Two rat models of prostate cancer were used; MAT-LyLu tumours which grow in Copenhagen rats and PAIII tumours which grow in Lobu nd-Wistar rats, with crossover of the cell lines to test allogeneic vaccina tion. The cell lines were immunologically characterized by flow cytometry. Irradiated tumour cells were administered as subcutaneous vaccines either b efore tumour challenge or after tumour establishment (both subcutaneous). A preparation of heat-killed Mycobacterium vaccae bacilli (SRL172) was used as an adjuvant to increase vaccine efficiency. Results Flow cytometry analysis of the cell lines showed that the PAIII cel ls had higher levels of major histocompatibility complex (MHC) class I and intercellular adhesion molecule (ICAM-1) expression than the MAT-LyLu cells . However, both tumour cell lines were rejected in their allogeneic hosts. Prophylactic vaccination with allogeneic MAT-LyLu cells protected against P AIII tumour challenge in Lobund-Wistar rats, with 80% of animals surviving for > 5 months, compared with 40% for animals receiving autologous cells. T he immunity was prolonged, as rats were protected when rechallenged 5 month s later. In Copenhagen rats allogeneic PAIII cells protected against the mo re aggressive MAT-LyLu tumour challenge only when the cells were combined w ith SRL172. Initial therapy experiments showed that vaccination with the ce ll lines mediated only limited tumour regression in the Lobund-Wistar rats. Conclusion The allogeneic tumour cell vaccination model described is valuab le for assessing the principle and efficacy of allogeneic prostate cancer c ell vaccines for clinical use.