Disposable versus reusable biopsy forceps for colorectal epithelial cell proliferation in humans

The performance of various measures of rectal mucosal proliferation has bee n evaluated in the literature, but the performance of the forceps used to o btain the tissue has received little attention. We used data from two large studies of proliferation at a single institution to compare reusable and d isposable endoscopic forceps. Endoscopic pinch biopsies were taken 10 cm fr om the anal verge using either reusable or disposable, oval-cupped, sheathe d forceps, The specimens were fixed, embedded, and sectioned, taking care t o orient the specimens longitudinally, Five sections were placed on each sl ide. We determined how many slides did not contain eight scorable crypts (i nadequate) and how many sections were necessary to identify eight complete crypts, There were 395 subjects who had biopsies taken with reusable forcep s and 185 subjects who had biopsies taken with disposable forceps. The spec imens were inadequate in 27.6% of the reusable forceps specimens versus 2.7 % of the disposable forceps (P < 0.0001), The mean number of tissue section s necessary to identify eight scorable crypts for the reusable forceps was 3.82 (SD, 0.87) compared with 3.17 (SD, 0.83) for disposable forceps (P = 0 .0001), The specimens taken with the disposable forceps were better, probab ly because the forceps were sharper. We believe that the better quality of the specimens and the sterility justify the higher cost of disposable force ps. We would urge investigators in proliferation studies to evaluate the bi opsy equipment as carefully as they evaluate other aspects of their methods .