Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata

Citation
R. Erra-basells et al., Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry of sulfated mannans from the red seaweed Nothogenia fastigiata, CARBOHY RES, 329(1), 2000, pp. 157-167
Citations number
23
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
329
Issue
1
Year of publication
2000
Pages
157 - 167
Database
ISI
SICI code
0008-6215(20001020)329:1<157:MULITM>2.0.ZU;2-P
Abstract
Matrix-assisted ultraviolet laser-desorption ionization time-of-flight mass spectrometry (UV-MALDI-TOF-MS) was applied to sulfated xylo-mannan fractio ns from Nothogenia fastigiata in order to determine their molecular weights and distribution profiles. The number-average molecular weight calculated from the spectra was similar to that determined by chemical end-group analy sis for the lower molecular weight fractions. For the other fractions, the number-average molecular weight was lower than that chemically determined; the increased difference may be attributed to higher desorption difficultie s and, consequently, mass-dependent discrimination. A reconstructed spectru m, using the peaks obtained from all the fractions, suggested an unimodal d istribution. The best results were obtained by using 2,5-dihydroxybenzoic a cid as matrix doped with 1-hydroxyisoquinoline and with harmane and nor-har mane. (C) 2000 Elsevier Science Ltd. All rights reserved.