Amperometric enzymatic detection of phenols for HPLC

Tyrosinase, laccase and coconut tissue were compared as active biocomponent s in enzymatic detection of phenols. The obtained selectivity of biocatalyt ic activity of tyrosinase was found to be dependent on the way of enzyme im mobilisation. The measurements were carried out in flow-injection systems w ith flow-through reactors and carbon paste based integrated biosensors. For the use as a detector in a HPLC system the tyrosinase carbon paste bio sen sor was found to be the most convenient. The obtained detection limits were 0.072, 0.037 and 0.032 mg l(-1) for hydroquinone, phenol and catechol, res pectively.