DEVELOPMENT OF AN ELISA FOR CHUM SALMON (ONCORHYNCHUS-KETA) GROWTH-HORMONE

A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of Low levels of serum growth hormone ( GH) in chum salmon (Oncorhynchus keta). The antiserum to GH (a-rsGH) w as obtained from a rabbit immunized with recombinant chum salmon GH. T he noncompetitive ELISA was performed by a sandwich method using a-rsG H rabbit IgG as the first antibody, its biotinylated Fab' fragment as the second antibody, and the avidin-biotin reaction for signal amplifi cation. This assay could he run in 3 days and routinely detected GH at concentrations as low as 0.5 ng/ml. The development of an ELISA for C hi made possible quantification of serum GH levels. In this assay syst em, parallel dilution curves were obtained using purified chum salmon GH and GH's from several species of the genus Oncorhynchus. (C) 1997 E lsevier Science Inc.