Characterization of two fly LGR (leucine-rich repeat-containing, G protein-coupled receptor) proteins homologous to vertebrate glycoprotein hormone receptors: Constitutive activation of wild-type fly LGR1 but not LGR2 in transfected mammalian cells

The receptors for lutropin (LH), FSH, and TSH belong to the large G protein -coupled receptor (GPCR) superfamily and are unique in having a large N-ter minal extracellular (ecto-) domain important for interactions with the larg e glycoprotein hormone ligands. Recent studies indicated the evolution of a large family of the leucine-rich repeat-containing, G protein-coupled rece ptors (LGRs) with at least seven members in mammals. Based on the sequences of mammalian glycoprotein hormone receptors, we have identified a new LGR in Drosophila melanogaster and named it as fly LGR2 to distinguish it from the previously reported fly LH/FSH/TSH receptor (renamed as ny LGR1). Genom ic analysis indicated the presence of 10 exons in fly LGR2 as compared with 16 exons in fly LGR1. The deduced fly LGR2 complementary DNA (cDNA) showed 43 and 64% similarity to the fly LGR1 in the ectodomain and transmembrane region, respectively. Comparison of 12 LGRs from diverse species indicated that these proteins can be divided into three subfamilies and fly LGR1 and LGR2 belong to different subfamilies. Potential signaling mechanisms were t ested in human 293T cells overexpressing the fly receptors, Of interest, fl y LGR1, but not LGR2, showed constitutive activity as reflected by elevated basal cAMP production in transfected cells. The basal activity of fly LGR1 was further augmented following point mutations of key residues in the int racellular loop 3 or transmembrane VI, similar to those found in patients w ith familial male precocious puberty. The present study reports the cloning of fly LGR2 and indicates that the G protein-coupling mechanism is conserv ed in fly LGR1 as compared with the mammalian glycoprotein hormone receptor s. The characterization of fly receptors with features similar to mammalian glycoprotein hormone receptors allows a better understanding of the evolut ion of this unique group of GPCRs and future elucidation of their ligand si gnaling mechanisms.