REPRODUCIBILITY OF THE NONLINEAR-REGRESSION FIT TO THE FTIR SPECTRA OF LYSOZYME

Citation
Tf. Kumosinski et al., REPRODUCIBILITY OF THE NONLINEAR-REGRESSION FIT TO THE FTIR SPECTRA OF LYSOZYME, Talanta, 44(8), 1997, pp. 1441-1445
Citations number
8
Journal title
Talanta
ISSN journal
00399140 → ACNP
Volume
44
Issue
8
Year of publication
1997
Pages
1441 - 1445
Database
ISI
SICI code
0039-9140(1997)44:8<1441:ROTNFT>2.0.ZU;2-V
Abstract
Controversy exists concerning the influence of experimental artifacts on the number of component FTIR vibrational bands which may be resolve d from the amide I and II envelopes of proteins in water. Whether thes e bands represent unique populations of vibrating protein groups in a particular global 2 degrees structure or whether the bands are due to instrumental and environmental fluctuations has been addressed, T.F. K umosinski and J.J. Unruh, Talanta, 43 (1996) 199-219. The repeatabilit y of the methodology and the apparent uniqueness of the nonlinear regr ession fits are addressed in this study. We obtained a series of the s pectra of lysozyme, and carried-out nonlinear regression analysis of e ach spectrum. Coefficients of variation (COV) were calculated for the wavenumber and area values of assigned component peaks obtained. Low C OVs obtained attest to the precision of the methodology and the appare nt uniqueness of the nonlinear regression fits. This methodology for a cquisition and analysis of protein FTIR spectra yields results with go od precision. (C) 1997 Elsevier Science B.V.