ANALYTICAL ASSESSMENT OF THE OSCILLATING CHEMICAL-REACTIONS BY USE CHEMILUMINESCENCE DETECTION

This paper introduces the chemiluminescence (CL) detection in oscillat ing reaction-based determinations using the analyte pulse perturbation technique, a straightforward and expeditious approach to deriving qua ntitative analytical information from oscillating chemical reactions. The behavior of the H2O2-KSCN-CuSO4-NaOH oscillating system in the pre sence of luminol was examined by using the proposed detection method a nd the classical potentiometric technique. Some analytical and practic al aspects of both detection systems are discussed. A new analytical m ethod for the determination of vitamin Bg based on the sequential pert urbation produced by different amounts of this substance on the oscill ating chemical system was also developed in order to assess the potent ial of CL detection for routine analyses. The calibration curve thus o btained was linear over the range 0.5-2.0 mu mol of vitamin B-6, and t he precision and throughput were also quite good (3.04% as RSD and nin e samples h(-1), respectively). The proposed method was validated by d etermining the vitamin in pharmaceutical preparations. (C) 1997 Elsevi er Science B.V.