Developmentally induced changes in rat lung malic enzyme activities

To determine lung malic enzyme activity at varying stages of development, b oth cytosolic and mitochondrial enzyme activities were assayed in rat lungs at various stages from day 16 of fetal life to 2 months of postnatal life by measuring the pl production of (CO2)-C-14 from C-14-malate. Malic enzyme activities were significantly higher in the mitochondrial than in the cyto solic fractions at all ages studied. The mitochondrial malic enzyme activit y was significantly higher in canalicular stage (days 19-20) stage of lung development when compared to the glandular stage (days 16-18). The mitochon drial fraction at day 19 exhibited biphasic kinetics: high affinity, K-m = 0.45 mmol, V-max = 10.04 nmol/mg protein/min; and low affinity, K-m = 5.48 mmol, V-max = 56.83 nmol/mg protein/min. The cytosolic malic enzyme activit y of all fetal stages (saccular stage [days 16-18], canalicular stage [days 19-20], and glandular stage [days 21-22] were significantly higher when co mpared to postnatal levels (postnatal days 1-10 adult). In contrast to the mitochondrial fraction, at day 19, the cytosolic fraction showed a single K -m of 0.23 mmol, V-max = 12.32 nmol/mg protein/min. The increased mitochond rial malic enzyme activity during late gestation would suggest that, as we have previously demonstrated, anaplerotic substrates other than glucose, ma y provide a significant energy source in fetal lung The increased cytosolic activity in the prenatal phases would suggest that the NADPH provided from malic enzyme is an important co contributor to de novo fatty acid synthesi s, leading to surfactant synthesis, critical to normal lung development in late gestation.