Two experimental high rate algal ponds (HRAPs) (1.5 m(2), 570 L per unit),
each with a secondary clarifier for algal biomass separation (0.025 m(2), w
ithout recirculation), were fed with urban wastewater for a one-year period
(June 1993 to July 1994). The HRAPs were installed on the roof of the Depa
rtment of Hydraulic, Coastal and Environmental Engineering of the Technical
University of Catalonia, Barcelona, Spain (lat. 41 degrees 24' 42 " N; lon
g. 2 degrees 7' 42 " E). Nitrogen removal efficiency and changes in total n
itrogen, total organic nitrogen, NH4+-N, and oxidized nitrogen under differ
ent hydraulic retention times (HRTs) were compared. HRAP A was always opera
ted at a higher HRT than HRAP B. Both HRAPs were subjected to the same envi
ronmental conditions of solar radiation, air temperature and influent water
quality. Grab samples of influent, effluent of the HRAP (mixed liquor) and
final effluent from the clarifiers were taken once a week. The annual aver
age nitrogen removal was 73% for HRAP A, and 57% for HRAP B. Higher removal
in HRAP A was due to a lower inorganic nitrogen concentration in its efflu
ent. Significant differences (p < 0.05) in the relative proportions of nitr
ogen forms between the two HRAPs were observed only in autumn and winter. T
his was mainly because HRAP B did not achieve a high level of NH4+-N remova
l by stripping and algal uptake, as observed in HRAP A. NH4+-N stripping wa
s the most important mechanism for nitrogen removal (mean efficiency of 47%
and 32% in HRAP A and B, respectively) followed by algal uptake, and subse
quent algal separation in the clarifiers (mean efficiency of 26% and 25% in
HRAP A and B respectively). The conclusion of this study is that HRT deter
mines both the nitrogen removal efficiency and the distribution of nitrogen
forms in the effluent of a HRAP. The nitrogen removal level can be control
led through suitable HRT operating strategies. By operating at a HRT of 4 d
ays in spring and summer, and 10 days in autumn and winter, nitrogen concen
tration in the effluent of a HRAP system can be reduced to less than 15 mg
L-1 N.