Am. Enlund et D. Westerlund, Effects of aliphatic amines on capillary electrochromatographic performance of tricyclic antidepressants on octadecylsilica, J CHROMAT A, 895(1-2), 2000, pp. 17-25
Adding aliphatic amines to the mobile phase improves peak symmetry and effi
ciency in capillary electrochromatography of tricyclic antidepressants on o
ctadecylsilica. The most hydrophobic aliphatic amine studied, dimethyloctyl
amine (DMOA), was the most efficient. Despite the fact that the amine addit
ives substantially reduced the electroosmotic flow, the retention of the an
alytes decreased indicating a strong competitive effect of the additives. D
MOA gave the largest retention decrease, and simultaneously reduced the res
olution, indicating that silanophilic interaction is significant to the sep
aration. Highest efficiencies were obtained at the lowest pH (2.8). Acetoni
trile influenced both efficiency and peak symmetry, and best results were o
btained at 60%. (C) 2000 Elsevier Science B.V. All rights reserved.