Capillary Electrophoresis analysis of gentamicin sulphate with UV detection after pre-capillary derivatization with 1,2-phthalic dicarboxaldehyde andmercaptoacetic acid
E. Kaale et al., Capillary Electrophoresis analysis of gentamicin sulphate with UV detection after pre-capillary derivatization with 1,2-phthalic dicarboxaldehyde andmercaptoacetic acid, J CHROMAT A, 895(1-2), 2000, pp. 67-79
A selective, sensitive, and rapid pre-capillary derivatization method for d
etermination of the multicomponent aminoglycoside antibiotic gentamicin is
described. The derivatization reagents 1,2-phthalic dicarboxaldehyde and me
rcaptoacetic acid were used and the thioisoindole derivative was UV detecte
d at 330 nm. A central composite experimental design was performed to optim
ize selectivity and derivatization conditions. Baseline separation of genta
micin C-1, C-1a, C-2, C-2a, C-2b, sisomicin and several minor components wa
s achieved with a background electrolyte containing 30 mM sodium tetraborat
e, 7.5 mM beta -cyclodextrin and 12.5% (v/v) methanol at pH 10. Quantitativ
e analysis was performed and illustrated the potential use of capillary ele
ctrophoresis for the identification and quantitation of gentamicin as an al
ternative to methods prescribed in the United States Pharmacopeia and Europ
ean Pharmacopoeia. (C) 2000 Elsevier Science B.V. All rights reserved.