Enantioseparation of anionic analytes by non-aqueous capillary electrophoresis using quinine and quinidine derivatives as chiral counter-ions

A non-aqueous capillary electrophoretic method developed for the enantiosep aration of N-protected amino acids has been applied to the investigation of five new quinine and quinidine derivatives as chiral selectors: 1-adamanty l carbamoylated quinine, 3,4-dichlorophenyl carbamoylated quinidine, allyl carbamoylated dihydroquinine, allyl carbamoylated dihydroquinidine and 1-me thyl quininium iodide. The composition of the background electrolyte was 12 .5 mM ammonia, 100 mM octanoic acid in an ethanol-methanol (60:40 v/v) mixt ure containing a 10 mM concentration of the chiral selector. Under these co nditions, the enantioseparation of a series of various benzoyl, 3,5-dinitro benzoyl and 3,5-dinitrobenzyloxycarbonyl amino acid derivatives was studied with respect to selectand-selector relationship and enantioselectivity. (C ) 2000 Elsevier Science B.V. All rights reserved.