The wound healing properties of Channa striatus-cetrimide cream-wound contraction and glycosaminoglycan measurement

Haruan has been proved to influence the different phases of wound healing p rocess. The current research focuses on the effects of haruan on the differ ent constituents of extracellular matrix of healing wounds in normal and di abetic rats. Anaesthetized normal and streptozotocin induced diabetic rats were provided with excision wounds at the back and then animals were divide d into four groups as: group 1, wounds treated with cetrimide + haruan crea m; group 2, wounds treated with haruan cream; group 3, wounds treated with cetrimide (commercial) cream and group 4, wounds untreated and served as co ntrol. Animals were sacrificed after 3, 6, 9 and 12 days. These wounds were used to determine the hexosamine, protein, uronic acid and glycosaminoglyc an contents and the wound contraction. The results suggested a marked incre ase (P < 0.05) in the uronic acid, hexosamine and dermatan sulfate contents on day 3 of group 1 when compared with groups 2-4. Wound contraction of gr oup 1 was also markedly enhanced of group 1 (P < 0.01) when compared with g roups 2-4; On the basis of these results, we conclude that haruan enhances the synthesis of different glycosaminoglycans in healing wounds, which are the first component of extracellular matrix to be synthesized during the wo und healing process. The enhanced levels of glycosaminoglycans may help in the formation of a resistant scar and enhanced wound contraction represents the positive influence of haruan on the fibroplastic phase of wound healin g. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.