Mesoporous MCM-41 having well ordered long-range structure, large pore diam
eters, narrow pore-size distribution, high pore volume and specific surface
area has been synthesized. The surface of MCM-41 has an abundance of weakl
y acidic hydroxyl groups. Assay results show that MCM-41 is a more effectiv
e support for the immobilization of Penicillin Acylase (PA) than many of ot
her supports due to its structural and surface characteristics. PA can be i
mmobilized on MCM-41 through either direct immobilization or covalent coupl
ing. The former gives higher activity of IME than the later. In the direct
immobilization, PA molecules are immobilized on MCM-41 through the hydrogen
-bonded interaction between hydroxyl groups of MCM-41 and carbonyl or amino
groups in the PA molecule. (C) 2000 Elsevier Science B.V. All rights reser
ved.