Molecular differentiation of three species of Metagonimus by simple sequence repeat anchored polymerase chain reaction (SSR-PCR) amplification

Authors
Yang, HJ Guk, SM Han, ET Chai, JY
Citation
Hj. Yang et al., Molecular differentiation of three species of Metagonimus by simple sequence repeat anchored polymerase chain reaction (SSR-PCR) amplification, J PARASITOL, 86(5), 2000, pp. 1170-1172
Citations number
14
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF PARASITOLOGY
ISSN journal
00223395 → ACNP
Volume
86
Issue
5
Year of publication
2000
Pages
1170 - 1172
Database
ISI
SICI code
0022-3395(200010)86:5<1170:MDOTSO>2.0.ZU;2-5
Abstract
Genomic DNAs from 3 species of Metagonimus in the Republic of Korea, M. yok ogawai, M. miyatai, and M. takahashii, were amplified by simple sequence re peat anchored polymerase chain reaction. Primers of 3' or 5' termini of the (CA), repeats were used; 3 kinds of 3' anchored primers (CA)(8)RG, (CA)(4) RG, and (CA)(8)RY, and 2 kinds of 5' anchored primers BDB(CA)(7)C and DBDA( CA)(7). The results showed distinguishable banding patterns among the 3 spe cies of Metagonimus, suggesting that they have different genotypes.