Analysis of intestinal adaptation gene expression by cDNA expression arrays

Background: As a tool for determining gene expression on a genomic scale, c DNA microarrays are a promising new technology that can be applied to the s tudy of complex physiologic processes. The objective of this study was to c haracterize the expression of individual genes and patterns of gene express ion that might provide insight into the mechanism of intestinal adaptation after massive small bowel resection. Methods: Male ICR mice underwent a 50% proximal small bowel resection (SBR) or sham operation. After 3 days, the remnant ileum was harvested, weighed, and RNA extracted. Changes in gene ex pression were detected utilizing Clontech Atlas mouse cDNA expression array s. Some of these changes were confirmed by reverse transcriptase-polymerase chain reactions (RT-PCR) and Northern blots. Results: Analysis of these cD NA arrays revealed changes in the expression of multiple genes, including t hose involved in cell cycle regulation, apoptosis, DNA synthesis, and trans criptional regulation. The patterns of expression were consistent with the increased cell proliferation and apoptosis observed during intestinal adapt ation. A large number of genes not previously associated with intestinal ad aptation were identified. Conclusions: This technology may facilitate the e lucidation of the intricate cellular mechanisms underlying intestinal adapt ation.