V. Kapchina-toteva et al., Role of phenylurea cytokinin CPPU in apical dominance release in in vitro cultured Rosa hybrida L., J PL GR REG, 19(2), 2000, pp. 232-237
The effect of purine (BA) and phenylurea (CPPU) cytokinins on apical domina
nce release in in vitro cultured Rosa hybrida L., cv. Madelon and Motrea wa
s evaluated. Cv. Madelon shows stronger natural apical growth and fewer bra
nches than cv. Motrea in vivo and in vitro. We examined the effects under t
hree conditions, without the addition of the auxin IBA, in the presence of
IBA, and in material pretreated with a pulse of IBA. Results were scored we
ekly for 4 weeks. BA and CPPU stimulated axillary bud break, and higher num
bers of open buds were recorded in the presence of CPPU. When CPPU cytokini
n was added to culture medium, physiologic features such as bud sprouting a
nd shoot fresh and dry weight were enhanced. CPPU was also highly efficient
for overcoming IBA inhibition of bud outgrowth. Different cultivar respons
es were observed.