Supercritical fluid extraction and chromatographic analysis (HRGC-FID and HRGC-MS) of Lupinus spp. alkaloids

The alkaloid extracts from Lupinus spp., obtained by conventional methods ( maceration/sonication - solid phase extraction; maceration/sonication - liq uid-liquid extraction) and SFE (supercritical fluid extraction) using CO2 a nd modified CO2 (CO2/MeOH, CO2/EtOH, CO2/(PrOH)-Pr-i and CO2/H2O) were anal ysed by HRGC-FID (high resolution gas chromatography - flame ionization det ector) and HRGC-MS (high resolution gas chromatography - mass spectrometry) . The HRGC-FID quantitative analyses were performed with an internal standa rd method for quantification of lupanine, multiflorine and a spartein-like alkaloid. HRGC-MS allowed identification of the chemical constituents (alka loids and other compounds) from these extracts.