Ac. Nossack et al., Supercritical fluid extraction and chromatographic analysis (HRGC-FID and HRGC-MS) of Lupinus spp. alkaloids, J BRAZ CHEM, 11(5), 2000, pp. 495-501
The alkaloid extracts from Lupinus spp., obtained by conventional methods (
maceration/sonication - solid phase extraction; maceration/sonication - liq
uid-liquid extraction) and SFE (supercritical fluid extraction) using CO2 a
nd modified CO2 (CO2/MeOH, CO2/EtOH, CO2/(PrOH)-Pr-i and CO2/H2O) were anal
ysed by HRGC-FID (high resolution gas chromatography - flame ionization det
ector) and HRGC-MS (high resolution gas chromatography - mass spectrometry)
. The HRGC-FID quantitative analyses were performed with an internal standa
rd method for quantification of lupanine, multiflorine and a spartein-like
alkaloid. HRGC-MS allowed identification of the chemical constituents (alka
loids and other compounds) from these extracts.