Mm. Chaves et al., Increase of reactive oxygen (ROS) and nitrogen (RNS) species generated by phagocyting granulocytes related to age, MECH AGE D, 119(1-2), 2000, pp. 1-8
In this present paper the age-induced effect on reactive oxidizing species
generated by oxygen (ROS) and nitrogen (RNS) was studied using human phagoc
yting granulocytes. The ROS and RNS were quantified, respectively, in a che
miluminescence assay and by the measurement of nitrite production. The age-
induced reactive oxidizing species generation was studied in healthy subjec
ts ranging from 20 to 80 years old, divided into six age groups: group I, 2
0-29 years old; group II, 30-39 years old; group III, 40-49 years old; grou
p IV, 50-59 years old; group V, 60-69 years old, and group VI, 70-80 years
old. Our results demonstrate a parallelism between generation of the ROS an
d RNS induced by the age. A significant increase of ROS production was obse
rved from 40 years old (age groups III, IV, V and VI while for RNS this inc
rease was observed only from 50 years old (groups IV, V and VI). These data
suggest an increase of oxidizing species generation (ROS/RNS) related to a
ge. The increased generation of ROS (40-49 years old) was induced before th
e increasing of RNS (50-59 years old) and it may have consequences on infla
mmation and host defences. (C) 2000 Elsevier Science Ireland Ltd. All right
s reserved.