Increase of reactive oxygen (ROS) and nitrogen (RNS) species generated by phagocyting granulocytes related to age

Citation
Mm. Chaves et al., Increase of reactive oxygen (ROS) and nitrogen (RNS) species generated by phagocyting granulocytes related to age, MECH AGE D, 119(1-2), 2000, pp. 1-8
Citations number
26
Categorie Soggetti
Cell & Developmental Biology
Journal title
MECHANISMS OF AGEING AND DEVELOPMENT
ISSN journal
00476374 → ACNP
Volume
119
Issue
1-2
Year of publication
2000
Pages
1 - 8
Database
ISI
SICI code
0047-6374(20001020)119:1-2<1:IORO(A>2.0.ZU;2-Q
Abstract
In this present paper the age-induced effect on reactive oxidizing species generated by oxygen (ROS) and nitrogen (RNS) was studied using human phagoc yting granulocytes. The ROS and RNS were quantified, respectively, in a che miluminescence assay and by the measurement of nitrite production. The age- induced reactive oxidizing species generation was studied in healthy subjec ts ranging from 20 to 80 years old, divided into six age groups: group I, 2 0-29 years old; group II, 30-39 years old; group III, 40-49 years old; grou p IV, 50-59 years old; group V, 60-69 years old, and group VI, 70-80 years old. Our results demonstrate a parallelism between generation of the ROS an d RNS induced by the age. A significant increase of ROS production was obse rved from 40 years old (age groups III, IV, V and VI while for RNS this inc rease was observed only from 50 years old (groups IV, V and VI). These data suggest an increase of oxidizing species generation (ROS/RNS) related to a ge. The increased generation of ROS (40-49 years old) was induced before th e increasing of RNS (50-59 years old) and it may have consequences on infla mmation and host defences. (C) 2000 Elsevier Science Ireland Ltd. All right s reserved.