Screening pedigrees for inherited minisatellite length changes provides an
efficient means of monitoring repeat DNA instability but has given rise to
apparently contradictory results regarding the effects of radiation on the
human germline. To explore this further in individuals with known radiation
doses and to potentially gain information on the timing of mutation induct
ion, we have used an extremely sensitive single molecule approach to quanti
fy the frequencies of mutation at the hypervariable minisatellites B6.7 and
CEB1 in the sperm of three seminoma patients following hemipelvic radiothe
rapy. Scattered radiation doses to the testicles were monitored and pre-tre
atment sperm DNA was compared with sperm derived from irradiated pre-meioti
c, meiotic and post-meiotic cells. We show no evidence for mutation inducti
on in any of the patients and discuss this finding in the context of previo
us population studies using minisatellites as reporter systems, one of whic
h provided evidence for radiation-induced germline mutation. (C) 2000 Elsev
ier Science B.V. All rights reserved.