Legume seeds (soy bean, lupin and bean seeds) were soaked in 0.5% sodium bi
carbonate in attempt to evaluate their nutritional quality and protein solu
bility index. Soaking process led to an increase in the hydration coefficie
nt, seed weight, total protein, ash, fat, fiber, while non protein nitrogen
, total carbohydrates, starch, stachyose, raffinose, reducing sugars, and m
inerals (except Na) were decreased. All antinutritional factors such as phy
tic acid, tannin, trypsin inhibitor and hemagglutinin activity were decreas
ed during soaking in 0.5% sodium bicarbonate; it was the same for the prote
in solubility in different solutions, while the in-vitro protein digestibil
ity and available lysine were increased.