Isolation and characterization of three polyamine oxidase genes from Zea mays

Isolation and sequencing of three genes, MPAO1, MPAO2 and MPAO3, coding for polyamine oxidase (PAO) from maize (Zea mays) are reported here. Gene orga nization is extremely conserved among these copies, being composed of eight exons and seven introns. Furthermore, these genes encode for a protein of an almost identical amino acid sequence. These data suggest that the three MPAO copies have been derived from gene duplication of a common ancestor ge ne. Long inverted repeat sequences, also present in other maize genes, have been found within the second intron. Promoter sequences of MPAO1 and MPAO2 genes have been analysed for putative cis-acting elements. According to ge nomic Southern blot analysis, the MPAO gene family in maize and other monoc ots is represented by a small number of copies. Northern and western blot a nalysis have revealed a tissue-specific accumulation of both MPAO mRNA and protein. (C) 2000 Editions scientifiques et medicales Elsevier SAS.