L. Simonetti et al., Estrus synchronization in ewes treated with sponges impregnated with different doses of medroxyprogesterone acetate, SMALL RUMIN, 38(3), 2000, pp. 243-247
The objectives of this study were to determine residual and absorbed levels
of medroxyprogesterone acetate (MAP) after treatment with intravaginal spo
nges impregnated with different doses of MAP in an ewe herd and to determin
e the effects of such treatments upon estrus incidence, interval to estrus
onset and pregnancy rate. Polyurethane sponges impregnated with 40, 50 and
60 mg of MAP were prepared. Real amounts of progestagen on sponges were che
cked prior to sponge treatment insertion. During autumn 1999, 608 cyclic Me
rino ewes were treated with intravaginal sponges impregnated with different
doses of MAP (groups I, 40; II, 50 and III, 60 mg). After 14 days, sponges
were removed and residual levels of MAP (RMAP) on removed sponges were mea
sured by spectrophotometry. Real amounts for unused sponges were 39.60+/-1.
10, 44.10+/-1.06 and 59.10+/-1.26 mg for the intended doses of 40, 50 and 6
0 mg MAP respectively. RMAPs were different (p<0.05) among groups (I: 17.98
+/-1.97 mg; II: 24.32+/-2.03 mg; III: 34.25+/-3.23 mg). Absorbed levels of
MAP were. not different among groups (I: 21.62+/-1.97 mg; II: 19.78+/-2.03
mg; III: 24.85+/-3.23 mg). Artificial insemination was performed in 16 ewes
from Group I, 17 ewes from Group II and 11 ewes from Group III, 12 h after
estrus onset. There were no differences among groups, neither for estrus i
ncidence (I: 79.27%; II: 77.42%; III: 80.87%) nor interval to estrus onset
(I: 55.94+/-1.87h; II: 56.74+/-1.13 h; III: 57.70+/-1.02 h). There were als
o no differences among groups for pregnancy rate (I: 43.75%; II: 52.94%; II
I: 45.45%). It was concluded that under similar conditions, a dose as low a
s 40 mg MAP could be effectively used for estrus synchronization in cyclic
Merino ewes. (C) 2000 Elsevier Science B.V. All rights reserved.