Novel goblet cell gene related to IgGFc gamma BP is regulated in adapting gut after small bowel resection

The loss of functional small bowel surface area leads to a well-described a daptive response in the remnant intestine. To elucidate its molecular regul ation, a cohort of cDNAs were cloned using a rat gut resection model and su btractive/differential hybridization cloning techniques. This study reports a novel cDNA termed "ileal remnant repressed" (IRR)-219, which shares 80% nucleotide identity with the 3' end of a human intestinal IgG Fc binding pr otein (IgGFc gamma BP) and is homologous to human and rat mucins. IRR-219 m RNA is expressed in intestine and colon only. At 48 h after 70% intestinal resection, mRNA levels decreased two- to fivefold in the adaptive small bow el but increased two- to threefold in the colon. Expression of IRR-219 was suppressed in adaptive small bowel as late as 1 wk after resection. IRR-219 expression is also regulated during gut ontogeny. In situ hybridization re vealed IRR-219 expression in small intestinal and colonic goblet cells only . Its unique patterns of expression during ontogeny and after small bowel r esection suggest distinctive roles in small bowel and colonic adaptation.