An enzyme-linked immunosorbent assay to quantify 14-3-3 proteins in the cerebrospinal fluid of suspected Creutzfeldt-Jakob disease patients

The detection of 14-3-3 protein by Western immunoblot is a sensitive and sp ecific cerebrospinal fluid marker of Creutzfeldt-Jakob disease (CJD). We de veloped a quantitative enzyme-linked immunosorbent assay (ELISA) that relia bly detects 14-3-3 in cerebrospinal fluid. In a prospective study of 147 ce rebrospinal fluid samples, the mean 14-3-3 concentration among pathological ly confirmed CJD patients (28.0 +/- 20.6 ng/ml, n = 41) is significantly hi gher than the mean in the cerebrospinal fluid of those with other neurologi cal disorders (3.1 +/- 2.9 ng/ml, n = 84). At a cutoff value of 8.3 ng/ml, the ELISA has a sensitivity of 92.7% and a specificity of 97.6%. The 14-3-3 ELISA supports a diagnosis of CJD in patients who fulfill clinical criteri a for possible CJD.