Identification of ubiquitin as an immunophilin

Authors
Davis, DL Soldin, SJ
Citation
Dl. Davis et Sj. Soldin, Identification of ubiquitin as an immunophilin, BIOC BIOP R, 277(2), 2000, pp. 325-329
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN journal
0006291X → ACNP
Volume
277
Issue
2
Year of publication
2000
Pages
325 - 329
Database
ISI
SICI code
0006-291X(20001022)277:2<325:IOUAAI>2.0.ZU;2-P
Abstract
We have identified an 8.4 kDa minor immunophilin from calf thymus and Jurka t T cells as ubiquitin. It binds tacrolimus and sirolimus with K-d's of 0.8 and 0.08 nM, respectively, The binding of this protein to cyclosporin A is negligible. Binding of tacrolimus to two commercial sources of ubiquitin, a bovine product, and a recombinant human ubiquitin, was also demonstrated after HPLC purification of the purchased preparations. (C) 2000 Academic Pr ess.